Understanding the functionality of multiplexed sensors in order to aid design and enhance performance

Multiplexed sensors enable simultaneous detection of disease biomarkers and environmental contaminants using a single test. Test results feature multiple signals, which if unclear, can result in misdiagnosis. This work studies the phenomenon of weakening signal intensities on multiplexed lateral flow tests. Results indicate that physical analyte entrapment has minimal impact on this phenomenon, which is influenced more, by the quantity of analyte in the sample and the positioning of the test line. Recommendations are made on the maximum number of test lines that should be employed to avoid reduced signal intensities, and the benefits of paper based microfluidics are discussed.