Tylosema esculentum extractives and their bioactivity

Ofentse Mazimba, Runner R.T. Majinda, Ceciliah Modibedi, Ishmael B. Masesane, Avrelija Cencič, Walter Chingwaru

Research output: Contribution to journalArticle

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Abstract

The investigation of Tylosema esculentum (Morama) husks, cotyledons, and tuber yielded griffonilide 2, compound 1, griffonin 3, gallic acid 4, protocatechuic acid 5, β-sitosterol 6, behenic acid 7, oleic acid 8, sucrose 9, 2-O-ethyl-α-d-glucopyranoside 10, kaempferol 11 and kaempferol-3-O-β-d-glucopyranoside 12. The structures of the isolates were determined by NMR, HR-TOF EIMS, IR and UV–vis spectroscopy, and by comparison with literature data. The husk EtOAc and n-butanol extracts demonstrated >90% DPPH radical scavenging activity at concentrations of 25, 50 and 250μg/mL. Furthermore the husk extracts showed higher total phenolic content (233mg GAE/g). The extractives exhibited minimum inhibitory quantities of 50–100μg or no activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Candida albicans. The tuber extracts were inactive against Caco-2 and Hela cell lines, while the husk extracts showed low activity against Caco-2 and Vero cell line with IC50 values >400μg/mL. The GC–MS analysis showed the beans and tuber non-polar (n-hexane) extracts major constituents as fatty acids.
Original languageEnglish
Pages (from-to)5225-5230
Number of pages6
JournalBioorganic and Medicinal Chemistry
Volume19
Issue number17
DOIs
Publication statusPublished - 2011

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Caco-2 Cells
Bioactivity
Cells
Cell Line
Gallic Acid
1-Butanol
Vero Cells
Cotyledon
Candida
Scavenging
Bacilli
Oleic Acid
Bacillus subtilis
Candida albicans
HeLa Cells
Gas Chromatography-Mass Spectrometry
Pseudomonas aeruginosa
Escherichia coli
Inhibitory Concentration 50
Sucrose

Cite this

Mazimba, O., Majinda, R. R. T., Modibedi, C., Masesane, I. B., Cencič, A., & Chingwaru, W. (2011). Tylosema esculentum extractives and their bioactivity. Bioorganic and Medicinal Chemistry, 19(17), 5225-5230. https://doi.org/10.1016/j.bmc.2011.07.006
Mazimba, Ofentse ; Majinda, Runner R.T. ; Modibedi, Ceciliah ; Masesane, Ishmael B. ; Cencič, Avrelija ; Chingwaru, Walter. / Tylosema esculentum extractives and their bioactivity. In: Bioorganic and Medicinal Chemistry. 2011 ; Vol. 19, No. 17. pp. 5225-5230.
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Mazimba, O, Majinda, RRT, Modibedi, C, Masesane, IB, Cencič, A & Chingwaru, W 2011, 'Tylosema esculentum extractives and their bioactivity', Bioorganic and Medicinal Chemistry, vol. 19, no. 17, pp. 5225-5230. https://doi.org/10.1016/j.bmc.2011.07.006

Tylosema esculentum extractives and their bioactivity. / Mazimba, Ofentse; Majinda, Runner R.T.; Modibedi, Ceciliah; Masesane, Ishmael B.; Cencič, Avrelija; Chingwaru, Walter.

In: Bioorganic and Medicinal Chemistry, Vol. 19, No. 17, 2011, p. 5225-5230.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Tylosema esculentum extractives and their bioactivity

AU - Mazimba, Ofentse

AU - Majinda, Runner R.T.

AU - Modibedi, Ceciliah

AU - Masesane, Ishmael B.

AU - Cencič, Avrelija

AU - Chingwaru, Walter

PY - 2011

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N2 - The investigation of Tylosema esculentum (Morama) husks, cotyledons, and tuber yielded griffonilide 2, compound 1, griffonin 3, gallic acid 4, protocatechuic acid 5, β-sitosterol 6, behenic acid 7, oleic acid 8, sucrose 9, 2-O-ethyl-α-d-glucopyranoside 10, kaempferol 11 and kaempferol-3-O-β-d-glucopyranoside 12. The structures of the isolates were determined by NMR, HR-TOF EIMS, IR and UV–vis spectroscopy, and by comparison with literature data. The husk EtOAc and n-butanol extracts demonstrated >90% DPPH radical scavenging activity at concentrations of 25, 50 and 250μg/mL. Furthermore the husk extracts showed higher total phenolic content (233mg GAE/g). The extractives exhibited minimum inhibitory quantities of 50–100μg or no activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Candida albicans. The tuber extracts were inactive against Caco-2 and Hela cell lines, while the husk extracts showed low activity against Caco-2 and Vero cell line with IC50 values >400μg/mL. The GC–MS analysis showed the beans and tuber non-polar (n-hexane) extracts major constituents as fatty acids.

AB - The investigation of Tylosema esculentum (Morama) husks, cotyledons, and tuber yielded griffonilide 2, compound 1, griffonin 3, gallic acid 4, protocatechuic acid 5, β-sitosterol 6, behenic acid 7, oleic acid 8, sucrose 9, 2-O-ethyl-α-d-glucopyranoside 10, kaempferol 11 and kaempferol-3-O-β-d-glucopyranoside 12. The structures of the isolates were determined by NMR, HR-TOF EIMS, IR and UV–vis spectroscopy, and by comparison with literature data. The husk EtOAc and n-butanol extracts demonstrated >90% DPPH radical scavenging activity at concentrations of 25, 50 and 250μg/mL. Furthermore the husk extracts showed higher total phenolic content (233mg GAE/g). The extractives exhibited minimum inhibitory quantities of 50–100μg or no activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Candida albicans. The tuber extracts were inactive against Caco-2 and Hela cell lines, while the husk extracts showed low activity against Caco-2 and Vero cell line with IC50 values >400μg/mL. The GC–MS analysis showed the beans and tuber non-polar (n-hexane) extracts major constituents as fatty acids.

U2 - 10.1016/j.bmc.2011.07.006

DO - 10.1016/j.bmc.2011.07.006

M3 - Article

VL - 19

SP - 5225

EP - 5230

JO - Bioorganic and Medicinal Chemistry

JF - Bioorganic and Medicinal Chemistry

SN - 0968-0896

IS - 17

ER -