Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 5225-5230 |
| Number of pages | 6 |
| Journal | Bioorganic and Medicinal Chemistry |
| Volume | 19 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - 2011 |
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Tylosema esculentum extractives and their bioactivity. / Mazimba, Ofentse; Majinda, Runner R.T.; Modibedi, Ceciliah; Masesane, Ishmael B.; Cencič, Avrelija; Chingwaru, Walter.
In: Bioorganic and Medicinal Chemistry, Vol. 19, No. 17, 2011, p. 5225-5230.Research output: Contribution to journal › Article
TY - JOUR
T1 - Tylosema esculentum extractives and their bioactivity
AU - Mazimba, Ofentse
AU - Majinda, Runner R.T.
AU - Modibedi, Ceciliah
AU - Masesane, Ishmael B.
AU - Cencič, Avrelija
AU - Chingwaru, Walter
PY - 2011
Y1 - 2011
N2 - The investigation of Tylosema esculentum (Morama) husks, cotyledons, and tuber yielded griffonilide 2, compound 1, griffonin 3, gallic acid 4, protocatechuic acid 5, β-sitosterol 6, behenic acid 7, oleic acid 8, sucrose 9, 2-O-ethyl-α-d-glucopyranoside 10, kaempferol 11 and kaempferol-3-O-β-d-glucopyranoside 12. The structures of the isolates were determined by NMR, HR-TOF EIMS, IR and UV–vis spectroscopy, and by comparison with literature data. The husk EtOAc and n-butanol extracts demonstrated >90% DPPH radical scavenging activity at concentrations of 25, 50 and 250μg/mL. Furthermore the husk extracts showed higher total phenolic content (233mg GAE/g). The extractives exhibited minimum inhibitory quantities of 50–100μg or no activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Candida albicans. The tuber extracts were inactive against Caco-2 and Hela cell lines, while the husk extracts showed low activity against Caco-2 and Vero cell line with IC50 values >400μg/mL. The GC–MS analysis showed the beans and tuber non-polar (n-hexane) extracts major constituents as fatty acids.
AB - The investigation of Tylosema esculentum (Morama) husks, cotyledons, and tuber yielded griffonilide 2, compound 1, griffonin 3, gallic acid 4, protocatechuic acid 5, β-sitosterol 6, behenic acid 7, oleic acid 8, sucrose 9, 2-O-ethyl-α-d-glucopyranoside 10, kaempferol 11 and kaempferol-3-O-β-d-glucopyranoside 12. The structures of the isolates were determined by NMR, HR-TOF EIMS, IR and UV–vis spectroscopy, and by comparison with literature data. The husk EtOAc and n-butanol extracts demonstrated >90% DPPH radical scavenging activity at concentrations of 25, 50 and 250μg/mL. Furthermore the husk extracts showed higher total phenolic content (233mg GAE/g). The extractives exhibited minimum inhibitory quantities of 50–100μg or no activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa and Candida albicans. The tuber extracts were inactive against Caco-2 and Hela cell lines, while the husk extracts showed low activity against Caco-2 and Vero cell line with IC50 values >400μg/mL. The GC–MS analysis showed the beans and tuber non-polar (n-hexane) extracts major constituents as fatty acids.
U2 - 10.1016/j.bmc.2011.07.006
DO - 10.1016/j.bmc.2011.07.006
M3 - Article
VL - 19
SP - 5225
EP - 5230
JO - Bioorganic and Medicinal Chemistry
JF - Bioorganic and Medicinal Chemistry
SN - 0968-0896
IS - 17
ER -