Strontium is incorporated into the fracture callus but does not influence the mechanical strength of healing rat fractures

Annemarie Brüel, Jakob Olsen, Henrik Birkedal, Malene Risager, Troels Torp Andreassen, Anders Christer Raffalt, Jens Enevold Thaulov Andersen, Jesper Skovhus Thomsen

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Strontium ranelate (SrR) is a new agent used in the treatment of osteoporosis and is suggested to reduce bone resorption and increase bone formation. We investigated whether SrR influences the macro-and nanomechnical properties of healing fractures in rats. A closed tibia fracture model was used to study fracture healing in rats after 3 and 8 weeks of healing. Two groups of rats were treated with SrR (900 mg/kg/day) mixed into the food, while two groups served as control animals. The healing fractures were investigated by three-point bending, dual energy X-ray absorptiometry, energy-dispersive X-ray spectroscopy (EDX), and nanoindentation. There was a 100-fold increase (P< 0.001) in serum Sr after 3 and 8 weeks of SrR treatment. The callus volume was significantly higher in the SrR-treated group than in control animals (P< 0.01) after 3 weeks of healing. This was accompanied by a significant increase in callus bone mineral content (P < 0.05). However, after 8 weeks of healing, no difference was found in either callus volume or bone mineral content. SrR did not influence maximum load or stiffness of the fractures after either 3 or 8 weeks of healing. EDX showed that Sr was incorporated into the callus; however, this did not influence the nanomechanical properties. In conclusion, SrR stimulates callus formation but has no effect on callus remodeling. Sr is incorporated into the newly formed callus tissue, but this has no deteriorating effect on the mechanical properties of rat tibial fractures at either the macroscopic or nanoscopic level after 3 or 8 weeks of healing.

Original languageEnglish
Pages (from-to)142-152
Number of pages11
JournalCalcified Tissue International
Volume88
Issue number2
DOIs
Publication statusPublished - Feb 1 2011

Fingerprint

strontium ranelate
Strontium
Fracture Healing
Bony Callus
X-Ray Emission Spectrometry
Bone Density
Closed Fractures
Tibial Fractures
Photon Absorptiometry
Bone Resorption
Tibia
Osteogenesis
Osteoporosis

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

Cite this

Brüel, A., Olsen, J., Birkedal, H., Risager, M., Andreassen, T. T., Raffalt, A. C., ... Thomsen, J. S. (2011). Strontium is incorporated into the fracture callus but does not influence the mechanical strength of healing rat fractures. Calcified Tissue International, 88(2), 142-152. https://doi.org/10.1007/s00223-010-9439-z
Brüel, Annemarie ; Olsen, Jakob ; Birkedal, Henrik ; Risager, Malene ; Andreassen, Troels Torp ; Raffalt, Anders Christer ; Andersen, Jens Enevold Thaulov ; Thomsen, Jesper Skovhus. / Strontium is incorporated into the fracture callus but does not influence the mechanical strength of healing rat fractures. In: Calcified Tissue International. 2011 ; Vol. 88, No. 2. pp. 142-152.
@article{9b0b7355cae549cb8716da0aa262dd69,
title = "Strontium is incorporated into the fracture callus but does not influence the mechanical strength of healing rat fractures",
abstract = "Strontium ranelate (SrR) is a new agent used in the treatment of osteoporosis and is suggested to reduce bone resorption and increase bone formation. We investigated whether SrR influences the macro-and nanomechnical properties of healing fractures in rats. A closed tibia fracture model was used to study fracture healing in rats after 3 and 8 weeks of healing. Two groups of rats were treated with SrR (900 mg/kg/day) mixed into the food, while two groups served as control animals. The healing fractures were investigated by three-point bending, dual energy X-ray absorptiometry, energy-dispersive X-ray spectroscopy (EDX), and nanoindentation. There was a 100-fold increase (P< 0.001) in serum Sr after 3 and 8 weeks of SrR treatment. The callus volume was significantly higher in the SrR-treated group than in control animals (P< 0.01) after 3 weeks of healing. This was accompanied by a significant increase in callus bone mineral content (P < 0.05). However, after 8 weeks of healing, no difference was found in either callus volume or bone mineral content. SrR did not influence maximum load or stiffness of the fractures after either 3 or 8 weeks of healing. EDX showed that Sr was incorporated into the callus; however, this did not influence the nanomechanical properties. In conclusion, SrR stimulates callus formation but has no effect on callus remodeling. Sr is incorporated into the newly formed callus tissue, but this has no deteriorating effect on the mechanical properties of rat tibial fractures at either the macroscopic or nanoscopic level after 3 or 8 weeks of healing.",
author = "Annemarie Br{\"u}el and Jakob Olsen and Henrik Birkedal and Malene Risager and Andreassen, {Troels Torp} and Raffalt, {Anders Christer} and Andersen, {Jens Enevold Thaulov} and Thomsen, {Jesper Skovhus}",
year = "2011",
month = "2",
day = "1",
doi = "10.1007/s00223-010-9439-z",
language = "English",
volume = "88",
pages = "142--152",
journal = "Calcified Tissue International",
issn = "0171-967X",
publisher = "Springer New York",
number = "2",

}

Strontium is incorporated into the fracture callus but does not influence the mechanical strength of healing rat fractures. / Brüel, Annemarie; Olsen, Jakob; Birkedal, Henrik; Risager, Malene; Andreassen, Troels Torp; Raffalt, Anders Christer; Andersen, Jens Enevold Thaulov; Thomsen, Jesper Skovhus.

In: Calcified Tissue International, Vol. 88, No. 2, 01.02.2011, p. 142-152.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Strontium is incorporated into the fracture callus but does not influence the mechanical strength of healing rat fractures

AU - Brüel, Annemarie

AU - Olsen, Jakob

AU - Birkedal, Henrik

AU - Risager, Malene

AU - Andreassen, Troels Torp

AU - Raffalt, Anders Christer

AU - Andersen, Jens Enevold Thaulov

AU - Thomsen, Jesper Skovhus

PY - 2011/2/1

Y1 - 2011/2/1

N2 - Strontium ranelate (SrR) is a new agent used in the treatment of osteoporosis and is suggested to reduce bone resorption and increase bone formation. We investigated whether SrR influences the macro-and nanomechnical properties of healing fractures in rats. A closed tibia fracture model was used to study fracture healing in rats after 3 and 8 weeks of healing. Two groups of rats were treated with SrR (900 mg/kg/day) mixed into the food, while two groups served as control animals. The healing fractures were investigated by three-point bending, dual energy X-ray absorptiometry, energy-dispersive X-ray spectroscopy (EDX), and nanoindentation. There was a 100-fold increase (P< 0.001) in serum Sr after 3 and 8 weeks of SrR treatment. The callus volume was significantly higher in the SrR-treated group than in control animals (P< 0.01) after 3 weeks of healing. This was accompanied by a significant increase in callus bone mineral content (P < 0.05). However, after 8 weeks of healing, no difference was found in either callus volume or bone mineral content. SrR did not influence maximum load or stiffness of the fractures after either 3 or 8 weeks of healing. EDX showed that Sr was incorporated into the callus; however, this did not influence the nanomechanical properties. In conclusion, SrR stimulates callus formation but has no effect on callus remodeling. Sr is incorporated into the newly formed callus tissue, but this has no deteriorating effect on the mechanical properties of rat tibial fractures at either the macroscopic or nanoscopic level after 3 or 8 weeks of healing.

AB - Strontium ranelate (SrR) is a new agent used in the treatment of osteoporosis and is suggested to reduce bone resorption and increase bone formation. We investigated whether SrR influences the macro-and nanomechnical properties of healing fractures in rats. A closed tibia fracture model was used to study fracture healing in rats after 3 and 8 weeks of healing. Two groups of rats were treated with SrR (900 mg/kg/day) mixed into the food, while two groups served as control animals. The healing fractures were investigated by three-point bending, dual energy X-ray absorptiometry, energy-dispersive X-ray spectroscopy (EDX), and nanoindentation. There was a 100-fold increase (P< 0.001) in serum Sr after 3 and 8 weeks of SrR treatment. The callus volume was significantly higher in the SrR-treated group than in control animals (P< 0.01) after 3 weeks of healing. This was accompanied by a significant increase in callus bone mineral content (P < 0.05). However, after 8 weeks of healing, no difference was found in either callus volume or bone mineral content. SrR did not influence maximum load or stiffness of the fractures after either 3 or 8 weeks of healing. EDX showed that Sr was incorporated into the callus; however, this did not influence the nanomechanical properties. In conclusion, SrR stimulates callus formation but has no effect on callus remodeling. Sr is incorporated into the newly formed callus tissue, but this has no deteriorating effect on the mechanical properties of rat tibial fractures at either the macroscopic or nanoscopic level after 3 or 8 weeks of healing.

UR - http://www.scopus.com/inward/record.url?scp=79954444751&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79954444751&partnerID=8YFLogxK

U2 - 10.1007/s00223-010-9439-z

DO - 10.1007/s00223-010-9439-z

M3 - Article

C2 - 21153023

AN - SCOPUS:79954444751

VL - 88

SP - 142

EP - 152

JO - Calcified Tissue International

JF - Calcified Tissue International

SN - 0171-967X

IS - 2

ER -