Simultaneous spectrophotometric determination of iron (ii) and copper (ii) in tablets by chemometric methods

Melisew Tadele Alula, Abdel Maaboud I. Mohamed, Adnan A. Bekhit

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The significant spectral overlap (Di)0.5 = 0.5667 which is about 75.3% overlapping of the UV/Visible absorption spectra of iron (II) and copper (II) complexes necessitates chemometric assisted methods for simultaneous analysis of these ions in the pharmaceutical mixture. These metal ions were analyzed simultaneously by UV/Visible spectrophotometric method where 8-hydroxyquinoline was used as a chromogenic reagent. The absorption spectra were recorded in the wavelength range of 350-600 nm digitized at 1 nm. Four chemometrics methods such as first derivative spectrophotometry (1D zero crossing), first derivative ratio (1D ratio), classical least squares (CLS) and principal component regression (PCR) were used to predict the concentrations of the metal ions. For the derivative spectrophotometry, measurements were made at two selected wavelengths. The calibration curves were linear in the range of 2-12 μg/ml for both ions. For classical least squares and principal component regression, the calibration model was obtained and predictions of the unknown concentrations of the ions in the synthetic mixtures and in the dosage form were done. The accuracy and the precision of these methods were determined by calculating the % recovery and the relative standard errors correspondingly. The methods were successfully applied for analyzing synthetic mixtures and commercial pharmaceutical preparation.

Original languageEnglish
Pages (from-to)93-106
Number of pages14
JournalThai Journal of Pharmaceutical Sciences
Volume34
Issue number3
Publication statusPublished - Jul 1 2010

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Tablets
Copper
Iron
Ions
Spectrophotometry
Least-Squares Analysis
Calibration
Metals
Oxyquinoline
Dosage Forms
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

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abstract = "The significant spectral overlap (Di)0.5 = 0.5667 which is about 75.3{\%} overlapping of the UV/Visible absorption spectra of iron (II) and copper (II) complexes necessitates chemometric assisted methods for simultaneous analysis of these ions in the pharmaceutical mixture. These metal ions were analyzed simultaneously by UV/Visible spectrophotometric method where 8-hydroxyquinoline was used as a chromogenic reagent. The absorption spectra were recorded in the wavelength range of 350-600 nm digitized at 1 nm. Four chemometrics methods such as first derivative spectrophotometry (1D zero crossing), first derivative ratio (1D ratio), classical least squares (CLS) and principal component regression (PCR) were used to predict the concentrations of the metal ions. For the derivative spectrophotometry, measurements were made at two selected wavelengths. The calibration curves were linear in the range of 2-12 μg/ml for both ions. For classical least squares and principal component regression, the calibration model was obtained and predictions of the unknown concentrations of the ions in the synthetic mixtures and in the dosage form were done. The accuracy and the precision of these methods were determined by calculating the {\%} recovery and the relative standard errors correspondingly. The methods were successfully applied for analyzing synthetic mixtures and commercial pharmaceutical preparation.",
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Simultaneous spectrophotometric determination of iron (ii) and copper (ii) in tablets by chemometric methods. / Alula, Melisew Tadele; Mohamed, Abdel Maaboud I.; Bekhit, Adnan A.

In: Thai Journal of Pharmaceutical Sciences, Vol. 34, No. 3, 01.07.2010, p. 93-106.

Research output: Contribution to journalArticle

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