Responses of meristematic callus cells of two Cynodon dactylon genotypes to aluminium

Sumita Ramgareeb, John A. Cooke, M. Paula Watt

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Responses to Al3+ of embryogenic callus cells of an Al-sensitive (Al-S) and Al-resistant (Al-R) Cynodon dactylon genotype were evaluated with regard to Al3+ toxicity and resistance. A chemical equilibrium speciation model (MINTEQA2) was used to ensure the availability of the Al 3+ ion in culture media, which was supplied as 0.08-2.3 mM Al 3+ for 2-8 weeks. Increasing Al3+ concentration and exposure time had a greater negative impact on the Al-S than on the Al-R genotype, in terms of callus growth rate and frequency of non-embryogenic cells. Exposure to 0.8 mM Al3+ for 2 weeks resulted in an 88% reduction in the Al-S meristematic cell number, whereas that of the Al-R genotype remained unaffected. In addition, the Al-S cells accumulated three times more Al in the nucleus than did the Al-R cells, suggesting that Al interfered with mitosis. The Al-R cells appeared to exclude Al3+ from its cells through an increase in extracellular pH (4.34 in Al-R and 4.08 in Al-S) and by the immobilisation of Al in the cell wall (33% more in Al-R). The results showed that by studying the cellular responses to Al3+ it is possible to discriminate between the Al-S and Al-R C. dactylon genotypes.

Original languageEnglish
Pages (from-to)1245-1258
Number of pages14
JournalJournal of Plant Physiology
Volume161
Issue number11
DOIs
Publication statusPublished - Jan 1 2004

Fingerprint

Cynodon
Bony Callus
Cynodon dactylon
Aluminum
aluminum
callus
Genotype
genotype
cells
chemical equilibrium
Mitosis
Immobilization
Cell Wall
Culture Media
mitosis
Cell Count
exposure duration
Ions
culture media
cell walls

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

@article{f5834f31d60c4efd8dde11b2ab2b1196,
title = "Responses of meristematic callus cells of two Cynodon dactylon genotypes to aluminium",
abstract = "Responses to Al3+ of embryogenic callus cells of an Al-sensitive (Al-S) and Al-resistant (Al-R) Cynodon dactylon genotype were evaluated with regard to Al3+ toxicity and resistance. A chemical equilibrium speciation model (MINTEQA2) was used to ensure the availability of the Al 3+ ion in culture media, which was supplied as 0.08-2.3 mM Al 3+ for 2-8 weeks. Increasing Al3+ concentration and exposure time had a greater negative impact on the Al-S than on the Al-R genotype, in terms of callus growth rate and frequency of non-embryogenic cells. Exposure to 0.8 mM Al3+ for 2 weeks resulted in an 88{\%} reduction in the Al-S meristematic cell number, whereas that of the Al-R genotype remained unaffected. In addition, the Al-S cells accumulated three times more Al in the nucleus than did the Al-R cells, suggesting that Al interfered with mitosis. The Al-R cells appeared to exclude Al3+ from its cells through an increase in extracellular pH (4.34 in Al-R and 4.08 in Al-S) and by the immobilisation of Al in the cell wall (33{\%} more in Al-R). The results showed that by studying the cellular responses to Al3+ it is possible to discriminate between the Al-S and Al-R C. dactylon genotypes.",
author = "Sumita Ramgareeb and Cooke, {John A.} and {Paula Watt}, M.",
year = "2004",
month = "1",
day = "1",
doi = "10.1016/j.jplph.2004.03.004",
language = "English",
volume = "161",
pages = "1245--1258",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "11",

}

Responses of meristematic callus cells of two Cynodon dactylon genotypes to aluminium. / Ramgareeb, Sumita; Cooke, John A.; Paula Watt, M.

In: Journal of Plant Physiology, Vol. 161, No. 11, 01.01.2004, p. 1245-1258.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Responses of meristematic callus cells of two Cynodon dactylon genotypes to aluminium

AU - Ramgareeb, Sumita

AU - Cooke, John A.

AU - Paula Watt, M.

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Responses to Al3+ of embryogenic callus cells of an Al-sensitive (Al-S) and Al-resistant (Al-R) Cynodon dactylon genotype were evaluated with regard to Al3+ toxicity and resistance. A chemical equilibrium speciation model (MINTEQA2) was used to ensure the availability of the Al 3+ ion in culture media, which was supplied as 0.08-2.3 mM Al 3+ for 2-8 weeks. Increasing Al3+ concentration and exposure time had a greater negative impact on the Al-S than on the Al-R genotype, in terms of callus growth rate and frequency of non-embryogenic cells. Exposure to 0.8 mM Al3+ for 2 weeks resulted in an 88% reduction in the Al-S meristematic cell number, whereas that of the Al-R genotype remained unaffected. In addition, the Al-S cells accumulated three times more Al in the nucleus than did the Al-R cells, suggesting that Al interfered with mitosis. The Al-R cells appeared to exclude Al3+ from its cells through an increase in extracellular pH (4.34 in Al-R and 4.08 in Al-S) and by the immobilisation of Al in the cell wall (33% more in Al-R). The results showed that by studying the cellular responses to Al3+ it is possible to discriminate between the Al-S and Al-R C. dactylon genotypes.

AB - Responses to Al3+ of embryogenic callus cells of an Al-sensitive (Al-S) and Al-resistant (Al-R) Cynodon dactylon genotype were evaluated with regard to Al3+ toxicity and resistance. A chemical equilibrium speciation model (MINTEQA2) was used to ensure the availability of the Al 3+ ion in culture media, which was supplied as 0.08-2.3 mM Al 3+ for 2-8 weeks. Increasing Al3+ concentration and exposure time had a greater negative impact on the Al-S than on the Al-R genotype, in terms of callus growth rate and frequency of non-embryogenic cells. Exposure to 0.8 mM Al3+ for 2 weeks resulted in an 88% reduction in the Al-S meristematic cell number, whereas that of the Al-R genotype remained unaffected. In addition, the Al-S cells accumulated three times more Al in the nucleus than did the Al-R cells, suggesting that Al interfered with mitosis. The Al-R cells appeared to exclude Al3+ from its cells through an increase in extracellular pH (4.34 in Al-R and 4.08 in Al-S) and by the immobilisation of Al in the cell wall (33% more in Al-R). The results showed that by studying the cellular responses to Al3+ it is possible to discriminate between the Al-S and Al-R C. dactylon genotypes.

UR - http://www.scopus.com/inward/record.url?scp=9644291394&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9644291394&partnerID=8YFLogxK

U2 - 10.1016/j.jplph.2004.03.004

DO - 10.1016/j.jplph.2004.03.004

M3 - Article

VL - 161

SP - 1245

EP - 1258

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

IS - 11

ER -