Physiological response and phytoremediation potential of Pinusthunbergii on Cd-contaminated Soil

Sim-Hee Han, DU-HYUN KIM, Venecio Ultra Jr

Research output: Contribution to journalArticle

Abstract

We investigated physiological responses and phytoextraction ability of Pinus thunbergii in cadmium contaminated soil as part of our efforts in identifying plant materials for the restoration and revegetation of forest soil contaminated by heavy metals. Thirty seedlings (ten per treatment) were assigned to three treatments (control, 0.3 and 0.6 mM CdSO 4 solution) at first year experiment. At second year, ten seedlings per treatment treated with Cd during the first year experiment were divided by two groups (no Cd-treated and consecutive Cd-treated group). At first experiment, photosynthetic pigment content, and superoxide dismutase (SOD) and glutathione reductase (GR) activities have significantly reduced by Cd application, and the reduction rate was increased much higher as the rate of Cd application increased. On the other hand, thiol and malondialdehyde (MDA) content were significantly increased at the application of 0.6mM of Cd. At the second year experiment, a general increase in chlorophyll and carotenoid content was observed with Cd treatment while SOD and GR activities showed a relative reduction compared to the control. Similar to the first year measurement, thiol and MDA contents also increased considerably due to Cd treatment. At harvest, dry matter was significantly reduced by Cd treatment especially at the rate of 0.6mM Cd, but dry yield of P. thunbergii treated with 0.3mM Cd was less affected and it was comparable with the control seeding. Cadmium concentration in seedling tissues increased with increasing Cd application rate while Cd uptake was higher in seedlings supplied with 0.3mM Cd which could be ascribe to their high dry matter. Overall, our study has demonstrated the unique physiological response of P. thunbergii to Cd-prolonged exposure by showing that the changes in photosynthetic pigment content and antioxidative enzyme activities were dependent on the concentration and duration of treatment. In addition, our results have demonstrated the potential of P. thunbergii to withstand up to 0.3mM Cd (equivalent to cumulative Cd concentration of 134.4 to 268 mg kg -1.
Original languageEnglish
Pages (from-to)711-719
JournalJournal of Korean Society of Forest Science
Volume99
Issue number5
Publication statusPublished - 2010

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Pinus thunbergii
phytoremediation
polluted soils
plant response
seedlings
glutathione-disulfide reductase
thiols
malondialdehyde
application rate
cadmium
superoxide dismutase
pigments
land restoration
physiological response
forest soils
carotenoids
heavy metals
sowing
enzyme activity
uptake mechanisms

Cite this

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title = "Physiological response and phytoremediation potential of Pinusthunbergii on Cd-contaminated Soil",
abstract = "We investigated physiological responses and phytoextraction ability of Pinus thunbergii in cadmium contaminated soil as part of our efforts in identifying plant materials for the restoration and revegetation of forest soil contaminated by heavy metals. Thirty seedlings (ten per treatment) were assigned to three treatments (control, 0.3 and 0.6 mM CdSO 4 solution) at first year experiment. At second year, ten seedlings per treatment treated with Cd during the first year experiment were divided by two groups (no Cd-treated and consecutive Cd-treated group). At first experiment, photosynthetic pigment content, and superoxide dismutase (SOD) and glutathione reductase (GR) activities have significantly reduced by Cd application, and the reduction rate was increased much higher as the rate of Cd application increased. On the other hand, thiol and malondialdehyde (MDA) content were significantly increased at the application of 0.6mM of Cd. At the second year experiment, a general increase in chlorophyll and carotenoid content was observed with Cd treatment while SOD and GR activities showed a relative reduction compared to the control. Similar to the first year measurement, thiol and MDA contents also increased considerably due to Cd treatment. At harvest, dry matter was significantly reduced by Cd treatment especially at the rate of 0.6mM Cd, but dry yield of P. thunbergii treated with 0.3mM Cd was less affected and it was comparable with the control seeding. Cadmium concentration in seedling tissues increased with increasing Cd application rate while Cd uptake was higher in seedlings supplied with 0.3mM Cd which could be ascribe to their high dry matter. Overall, our study has demonstrated the unique physiological response of P. thunbergii to Cd-prolonged exposure by showing that the changes in photosynthetic pigment content and antioxidative enzyme activities were dependent on the concentration and duration of treatment. In addition, our results have demonstrated the potential of P. thunbergii to withstand up to 0.3mM Cd (equivalent to cumulative Cd concentration of 134.4 to 268 mg kg -1.",
author = "Sim-Hee Han and DU-HYUN KIM and {Ultra Jr}, Venecio",
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journal = "Journal of Korean Society of Forest Science",
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Physiological response and phytoremediation potential of Pinusthunbergii on Cd-contaminated Soil. / Han, Sim-Hee; KIM, DU-HYUN; Ultra Jr, Venecio.

In: Journal of Korean Society of Forest Science, Vol. 99, No. 5, 2010, p. 711-719.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Physiological response and phytoremediation potential of Pinusthunbergii on Cd-contaminated Soil

AU - Han, Sim-Hee

AU - KIM, DU-HYUN

AU - Ultra Jr, Venecio

PY - 2010

Y1 - 2010

N2 - We investigated physiological responses and phytoextraction ability of Pinus thunbergii in cadmium contaminated soil as part of our efforts in identifying plant materials for the restoration and revegetation of forest soil contaminated by heavy metals. Thirty seedlings (ten per treatment) were assigned to three treatments (control, 0.3 and 0.6 mM CdSO 4 solution) at first year experiment. At second year, ten seedlings per treatment treated with Cd during the first year experiment were divided by two groups (no Cd-treated and consecutive Cd-treated group). At first experiment, photosynthetic pigment content, and superoxide dismutase (SOD) and glutathione reductase (GR) activities have significantly reduced by Cd application, and the reduction rate was increased much higher as the rate of Cd application increased. On the other hand, thiol and malondialdehyde (MDA) content were significantly increased at the application of 0.6mM of Cd. At the second year experiment, a general increase in chlorophyll and carotenoid content was observed with Cd treatment while SOD and GR activities showed a relative reduction compared to the control. Similar to the first year measurement, thiol and MDA contents also increased considerably due to Cd treatment. At harvest, dry matter was significantly reduced by Cd treatment especially at the rate of 0.6mM Cd, but dry yield of P. thunbergii treated with 0.3mM Cd was less affected and it was comparable with the control seeding. Cadmium concentration in seedling tissues increased with increasing Cd application rate while Cd uptake was higher in seedlings supplied with 0.3mM Cd which could be ascribe to their high dry matter. Overall, our study has demonstrated the unique physiological response of P. thunbergii to Cd-prolonged exposure by showing that the changes in photosynthetic pigment content and antioxidative enzyme activities were dependent on the concentration and duration of treatment. In addition, our results have demonstrated the potential of P. thunbergii to withstand up to 0.3mM Cd (equivalent to cumulative Cd concentration of 134.4 to 268 mg kg -1.

AB - We investigated physiological responses and phytoextraction ability of Pinus thunbergii in cadmium contaminated soil as part of our efforts in identifying plant materials for the restoration and revegetation of forest soil contaminated by heavy metals. Thirty seedlings (ten per treatment) were assigned to three treatments (control, 0.3 and 0.6 mM CdSO 4 solution) at first year experiment. At second year, ten seedlings per treatment treated with Cd during the first year experiment were divided by two groups (no Cd-treated and consecutive Cd-treated group). At first experiment, photosynthetic pigment content, and superoxide dismutase (SOD) and glutathione reductase (GR) activities have significantly reduced by Cd application, and the reduction rate was increased much higher as the rate of Cd application increased. On the other hand, thiol and malondialdehyde (MDA) content were significantly increased at the application of 0.6mM of Cd. At the second year experiment, a general increase in chlorophyll and carotenoid content was observed with Cd treatment while SOD and GR activities showed a relative reduction compared to the control. Similar to the first year measurement, thiol and MDA contents also increased considerably due to Cd treatment. At harvest, dry matter was significantly reduced by Cd treatment especially at the rate of 0.6mM Cd, but dry yield of P. thunbergii treated with 0.3mM Cd was less affected and it was comparable with the control seeding. Cadmium concentration in seedling tissues increased with increasing Cd application rate while Cd uptake was higher in seedlings supplied with 0.3mM Cd which could be ascribe to their high dry matter. Overall, our study has demonstrated the unique physiological response of P. thunbergii to Cd-prolonged exposure by showing that the changes in photosynthetic pigment content and antioxidative enzyme activities were dependent on the concentration and duration of treatment. In addition, our results have demonstrated the potential of P. thunbergii to withstand up to 0.3mM Cd (equivalent to cumulative Cd concentration of 134.4 to 268 mg kg -1.

M3 - Article

VL - 99

SP - 711

EP - 719

JO - Journal of Korean Society of Forest Science

JF - Journal of Korean Society of Forest Science

SN - 2586-6621

IS - 5

ER -