Persistence of antibiotic resistance and plasmid-associated genes in soil following application of sewage sludge and abundance on vegetables at harvest

Teddie O. Rahube, Romain Marti, Andrew Scott, Yuan Ching Tien, Roger Murray, Lyne Sabourin, Peter Duenk, David R. Lapen, Edward Topp

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Sewage sludge recovered from wastewater treatment plants contains antibiotic residues and is rich in antibiotic resistance genes, selected for and enriched in the digestive tracts of human using antibiotics. The use of sewage sludge as a crop fertilizer constitutes a potential route of human exposure to antibiotic resistance genes through consumption of contaminated crops. Several gene targets associated with antibiotic resistance (catA1, catB3, ereA, ereB, erm(B), str(A), str(B), qnrD, sul1, and mphA), mobile genetic elements (int1, mobA, IncW repA, IncP1 groups -α, -β, -σ, -γ, -ε), and bacterial 16S rRNA (rrnS) were quantified by qPCR from soil and vegetable samples obtained from unamended and sludge-amended plots at an experimental field in London, Ontario. The qPCR data reveals an increase in abundance of gene targets in the soil and vegetables samples, indicating that there is potential for additional crop exposure to antibiotic resistance genes carried within sewage sludge following field application. It is therefore advisable to allow an appropriate delay period before harvesting of vegetables for human consumption.

Original languageEnglish
Pages (from-to)600-607
Number of pages8
JournalCanadian Journal of Microbiology
Volume62
Issue number7
DOIs
Publication statusPublished - Mar 7 2016

Fingerprint

Microbial Drug Resistance
Sewage
Vegetables
Plasmids
Soil
Genes
Interspersed Repetitive Sequences
Anti-Bacterial Agents
Fertilizers
Ontario
Waste Water
Gastrointestinal Tract

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

Cite this

Rahube, Teddie O. ; Marti, Romain ; Scott, Andrew ; Tien, Yuan Ching ; Murray, Roger ; Sabourin, Lyne ; Duenk, Peter ; Lapen, David R. ; Topp, Edward. / Persistence of antibiotic resistance and plasmid-associated genes in soil following application of sewage sludge and abundance on vegetables at harvest. In: Canadian Journal of Microbiology. 2016 ; Vol. 62, No. 7. pp. 600-607.
@article{964247a743904c2b88ef733ddccb357c,
title = "Persistence of antibiotic resistance and plasmid-associated genes in soil following application of sewage sludge and abundance on vegetables at harvest",
abstract = "Sewage sludge recovered from wastewater treatment plants contains antibiotic residues and is rich in antibiotic resistance genes, selected for and enriched in the digestive tracts of human using antibiotics. The use of sewage sludge as a crop fertilizer constitutes a potential route of human exposure to antibiotic resistance genes through consumption of contaminated crops. Several gene targets associated with antibiotic resistance (catA1, catB3, ereA, ereB, erm(B), str(A), str(B), qnrD, sul1, and mphA), mobile genetic elements (int1, mobA, IncW repA, IncP1 groups -α, -β, -σ, -γ, -ε), and bacterial 16S rRNA (rrnS) were quantified by qPCR from soil and vegetable samples obtained from unamended and sludge-amended plots at an experimental field in London, Ontario. The qPCR data reveals an increase in abundance of gene targets in the soil and vegetables samples, indicating that there is potential for additional crop exposure to antibiotic resistance genes carried within sewage sludge following field application. It is therefore advisable to allow an appropriate delay period before harvesting of vegetables for human consumption.",
author = "Rahube, {Teddie O.} and Romain Marti and Andrew Scott and Tien, {Yuan Ching} and Roger Murray and Lyne Sabourin and Peter Duenk and Lapen, {David R.} and Edward Topp",
year = "2016",
month = "3",
day = "7",
doi = "10.1139/cjm-2016-0034",
language = "English",
volume = "62",
pages = "600--607",
journal = "Canadian Journal of Microbiology",
issn = "0008-4166",
publisher = "National Research Council of Canada",
number = "7",

}

Persistence of antibiotic resistance and plasmid-associated genes in soil following application of sewage sludge and abundance on vegetables at harvest. / Rahube, Teddie O.; Marti, Romain; Scott, Andrew; Tien, Yuan Ching; Murray, Roger; Sabourin, Lyne; Duenk, Peter; Lapen, David R.; Topp, Edward.

In: Canadian Journal of Microbiology, Vol. 62, No. 7, 07.03.2016, p. 600-607.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Persistence of antibiotic resistance and plasmid-associated genes in soil following application of sewage sludge and abundance on vegetables at harvest

AU - Rahube, Teddie O.

AU - Marti, Romain

AU - Scott, Andrew

AU - Tien, Yuan Ching

AU - Murray, Roger

AU - Sabourin, Lyne

AU - Duenk, Peter

AU - Lapen, David R.

AU - Topp, Edward

PY - 2016/3/7

Y1 - 2016/3/7

N2 - Sewage sludge recovered from wastewater treatment plants contains antibiotic residues and is rich in antibiotic resistance genes, selected for and enriched in the digestive tracts of human using antibiotics. The use of sewage sludge as a crop fertilizer constitutes a potential route of human exposure to antibiotic resistance genes through consumption of contaminated crops. Several gene targets associated with antibiotic resistance (catA1, catB3, ereA, ereB, erm(B), str(A), str(B), qnrD, sul1, and mphA), mobile genetic elements (int1, mobA, IncW repA, IncP1 groups -α, -β, -σ, -γ, -ε), and bacterial 16S rRNA (rrnS) were quantified by qPCR from soil and vegetable samples obtained from unamended and sludge-amended plots at an experimental field in London, Ontario. The qPCR data reveals an increase in abundance of gene targets in the soil and vegetables samples, indicating that there is potential for additional crop exposure to antibiotic resistance genes carried within sewage sludge following field application. It is therefore advisable to allow an appropriate delay period before harvesting of vegetables for human consumption.

AB - Sewage sludge recovered from wastewater treatment plants contains antibiotic residues and is rich in antibiotic resistance genes, selected for and enriched in the digestive tracts of human using antibiotics. The use of sewage sludge as a crop fertilizer constitutes a potential route of human exposure to antibiotic resistance genes through consumption of contaminated crops. Several gene targets associated with antibiotic resistance (catA1, catB3, ereA, ereB, erm(B), str(A), str(B), qnrD, sul1, and mphA), mobile genetic elements (int1, mobA, IncW repA, IncP1 groups -α, -β, -σ, -γ, -ε), and bacterial 16S rRNA (rrnS) were quantified by qPCR from soil and vegetable samples obtained from unamended and sludge-amended plots at an experimental field in London, Ontario. The qPCR data reveals an increase in abundance of gene targets in the soil and vegetables samples, indicating that there is potential for additional crop exposure to antibiotic resistance genes carried within sewage sludge following field application. It is therefore advisable to allow an appropriate delay period before harvesting of vegetables for human consumption.

UR - http://www.scopus.com/inward/record.url?scp=84977155298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84977155298&partnerID=8YFLogxK

U2 - 10.1139/cjm-2016-0034

DO - 10.1139/cjm-2016-0034

M3 - Article

AN - SCOPUS:84977155298

VL - 62

SP - 600

EP - 607

JO - Canadian Journal of Microbiology

JF - Canadian Journal of Microbiology

SN - 0008-4166

IS - 7

ER -