Normalization for cDNA microarray data of gene expression profiles from the human prostate cancer cell lines (PC3) by Pre-Processing Two-Color Data

Dorina Bratfalean, Ramona Suharoschi, Crina Muresan, S. P. Agachi, M. V. Cristea, Ana Maria Cormos, Àlex Gómez Garridoc, Miguel Hernández Sánchez

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The objective of the present work is normalization of cDNA microarray data of gene expression profiles from the human prostate cancer cell lines (PC3) treated with Genistein compared with untreated prostate cancer cells through pre-processing Two- Color Data. In this study, we utilized the high throughput gene chip, which contains 4486 known genes, to determine the alternation of gene expression profiles of PC3 prostate cancer cells exposed to Genistein at therapeutically and physiologically doses as well. The Limma was used to implement a range of normalization methods for spotted microarrays. Normalization was performed, for the experimental DNA microarray data, for three experimental groups, and the results show a very good accuracy.

Original languageEnglish
Title of host publication19th European Symposium on Computer Aided Process Engineering
EditorsJacek Jezowski, Jan Thullie
Pages1033-1038
Number of pages6
DOIs
Publication statusPublished - Jun 26 2009

Publication series

NameComputer Aided Chemical Engineering
Volume26
ISSN (Print)1570-7946

All Science Journal Classification (ASJC) codes

  • Chemical Engineering(all)
  • Computer Science Applications

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    Bratfalean, D., Suharoschi, R., Muresan, C., Agachi, S. P., Cristea, M. V., Cormos, A. M., Gómez Garridoc, À., & Hernández Sánchez, M. (2009). Normalization for cDNA microarray data of gene expression profiles from the human prostate cancer cell lines (PC3) by Pre-Processing Two-Color Data. In J. Jezowski, & J. Thullie (Eds.), 19th European Symposium on Computer Aided Process Engineering (pp. 1033-1038). (Computer Aided Chemical Engineering; Vol. 26). https://doi.org/10.1016/S1570-7946(09)70172-5