Monolayers of a de novo designed 4-α-helix bundle carboprotein and partial structures on Au(111)-surfaces

Jesper Brask, Hainer Wackerbarth, Knud J. Jensen, Jingdong Zhang, Jens U. Nielsen, Jens E.T. Andersen, Jens Ulstrup

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mapping of structure and function of proteins adsorbed on solid surfaces is important in many contexts. Electrochemical techniques based on single-crystal metal surfaces and in situ scanning probe microscopies (SPM) have recently opened new perspectives for mapping at the single-molecule level. De novo design of model proteins has evolved in parallel and holds promise for test and control of protein folding and for new tailored protein structural motifs. These two strategies are combined in the present report. We present a synthetic scheme for a new 4-α-helix bundle carboprotein built on a galactopyranoside derivative with a thiol anchor aglycon suitable for surface immobilization on gold. The galactopyranoside with thiol anchor and the thiol anchor alone were prepared for comparison. Voltammetry of the three molecules on Au(111) showed reductive desorption peaks caused by monolayer adsorption via thiolate-Au bonding. In situ STM of the thiol anchor disclosed an ordered adlayer with clear domains and molecular features. This holds promise, broadly for single-molecule voltammetry and the SPM and scanning tunnelling microscopy (STM) of natural and synthetic proteins.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalBioelectrochemistry
Volume56
Issue number1-2
DOIs
Publication statusPublished - May 15 2002

Fingerprint

Anchors
Sulfhydryl Compounds
helices
bundles
Monolayers
Scanning Probe Microscopy
thiols
Scanning Tunnelling Microscopy
proteins
Proteins
Scanning probe microscopy
Scanning tunneling microscopy
Voltammetry
Galactose
Molecules
Electrochemical Techniques
scanning tunneling microscopy
Protein folding
Amino Acid Motifs
Protein Folding

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Physical and Theoretical Chemistry
  • Electrochemistry

Cite this

Brask, Jesper ; Wackerbarth, Hainer ; Jensen, Knud J. ; Zhang, Jingdong ; Nielsen, Jens U. ; Andersen, Jens E.T. ; Ulstrup, Jens. / Monolayers of a de novo designed 4-α-helix bundle carboprotein and partial structures on Au(111)-surfaces. In: Bioelectrochemistry. 2002 ; Vol. 56, No. 1-2. pp. 27-32.
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Monolayers of a de novo designed 4-α-helix bundle carboprotein and partial structures on Au(111)-surfaces. / Brask, Jesper; Wackerbarth, Hainer; Jensen, Knud J.; Zhang, Jingdong; Nielsen, Jens U.; Andersen, Jens E.T.; Ulstrup, Jens.

In: Bioelectrochemistry, Vol. 56, No. 1-2, 15.05.2002, p. 27-32.

Research output: Contribution to journalArticle

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