Fungi, aflatoxins, fumonisin B1 and zearalenone contaminating sorghum-based traditional malt, wort and beer in Botswana

David O. Nkwe, Joanne E. Taylor, Bupe A. Siame

    Research output: Contribution to journalArticle

    28 Citations (Scopus)

    Abstract

    Brewing and consumption of traditional beer have social-economic significance in most African countries including Botswana. Traditional sorghum malt, wort, and beer samples were collected from three villages around Gaborone, Botswana. Forty-six malt samples were analyzed for fungi on three different media and developing colonies were subcultured for identification. Rhizopus, Fusarium, Mucor, and Aspergillus were the most common genera isolated. Out of the 46 malt samples, 72% contained Rhizopus stolonifer, 63% Fusarium verticillioides (syn. Fusarium moniliforme), and 37% Aspergillus flavus. Although Aspergillus flavus was isolated from malt samples, aflatoxins (B 1, B2, G1, and G2) were not detected in any of the samples analyzed. When the malt, wort, and beer samples were analyzed for fumonisin Bl and zearalenone, fumonisin B1 was detected in 3 malt samples, with concentrations ranging from 47 to 1316 μg/kg, while zearalenone was detected in 56%, 48% and 48% of the malt, wort and beer samples, respectively. Zearalenone concentration in samples ranged from 102 to 2213 μg/kg in malt, 26 to 285 μg/l in wort and 20 to 201 μg/l, in beer. Zearalenone carry-over from wort to beer ranged from 23 to 403%. Therefore, although aflatoxins and fumonisin B1 do not appear to be major contaminants, zearalenone is common and could pose a potential problem in traditional beer in Botswana.

    Original languageEnglish
    Pages (from-to)177-186
    Number of pages10
    JournalMycopathologia
    Volume160
    Issue number2
    DOIs
    Publication statusPublished - Jan 2005

    Fingerprint

    Botswana
    Zearalenone
    wort (brewing)
    Aflatoxin B1
    fumonisin B1
    Sorghum
    zearalenone
    malt
    beers
    aflatoxins
    Fungi
    fungi
    Fusarium
    Rhizopus
    Aspergillus flavus
    sampling
    Fumonisins
    Mucor
    Aspergillus
    Rhizopus stolonifer

    All Science Journal Classification (ASJC) codes

    • Agricultural and Biological Sciences (miscellaneous)
    • Plant Science

    Cite this

    @article{d68703e71d04463faacc76b60496f2cf,
    title = "Fungi, aflatoxins, fumonisin B1 and zearalenone contaminating sorghum-based traditional malt, wort and beer in Botswana",
    abstract = "Brewing and consumption of traditional beer have social-economic significance in most African countries including Botswana. Traditional sorghum malt, wort, and beer samples were collected from three villages around Gaborone, Botswana. Forty-six malt samples were analyzed for fungi on three different media and developing colonies were subcultured for identification. Rhizopus, Fusarium, Mucor, and Aspergillus were the most common genera isolated. Out of the 46 malt samples, 72{\%} contained Rhizopus stolonifer, 63{\%} Fusarium verticillioides (syn. Fusarium moniliforme), and 37{\%} Aspergillus flavus. Although Aspergillus flavus was isolated from malt samples, aflatoxins (B 1, B2, G1, and G2) were not detected in any of the samples analyzed. When the malt, wort, and beer samples were analyzed for fumonisin Bl and zearalenone, fumonisin B1 was detected in 3 malt samples, with concentrations ranging from 47 to 1316 μg/kg, while zearalenone was detected in 56{\%}, 48{\%} and 48{\%} of the malt, wort and beer samples, respectively. Zearalenone concentration in samples ranged from 102 to 2213 μg/kg in malt, 26 to 285 μg/l in wort and 20 to 201 μg/l, in beer. Zearalenone carry-over from wort to beer ranged from 23 to 403{\%}. Therefore, although aflatoxins and fumonisin B1 do not appear to be major contaminants, zearalenone is common and could pose a potential problem in traditional beer in Botswana.",
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    Fungi, aflatoxins, fumonisin B1 and zearalenone contaminating sorghum-based traditional malt, wort and beer in Botswana. / Nkwe, David O.; Taylor, Joanne E.; Siame, Bupe A.

    In: Mycopathologia, Vol. 160, No. 2, 01.2005, p. 177-186.

    Research output: Contribution to journalArticle

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