Effects of methyl bromide fumigation, chloropicrin fumigation and steam sterilization on soil nitrogen dynamics and microbial properties in a pot culture experiment

T. Yamamoto, V.U. Ultra Jr., S. Tanaka, K. Sakurai, K. Iwasaki

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The effects of steam sterilization (SS), methyl bromide (MeBr) fumigation and chloropicrin (CP) fumigation on soil N dynamics and microbial properties were evaluated in a pot experiment. All disinfection treatments increased the -N level and inhibited nitrification. The additional -N in the CP treatment probably originated from the decomposition of microbial debris by surviving microbes, while that in the SS treatment was attributable to deamination processes of soil organic N occurring in a less labile fraction in addition to the decomposition of microbial debris. The MeBr fumigation increased the level of -N without changing the soil microbial biomass. Based on the determinations of soil microbial biomass, substrate utilization activity (Biolog method) and microbial community structure (phospholipid fatty acid method), the effects of the MeBr, CP and SS treatments on the microbial community were compared. The MeBr fumigation had relatively mild and short-term effects on microbial biomass and activity, but altered the community structure drastically by promoting the growth of gram-positive bacteria. The CP fumigation had large and long-term impacts on microbial biomass and activity; the community structure remained unaffected except for the gram-negative bacteria. Steam sterilization had severe and persistent effects on all parameters. The severity of the effects decreased in the order SS ≥ CP > MeBr. © 2008 Japanese Society of Soil Science and Plant Nutrition.
Original languageEnglish
Pages (from-to)886-894
Number of pages9
JournalSoil Science and Plant Nutrition
Volume54
Issue number6
DOIs
Publication statusPublished - 2008

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chloropicrin
methyl bromide
sterilizing
pot culture
fumigation
soil nitrogen
steam
microbial biomass
nitrogen
community structure
biomass
soil
experiment
microbial activity
microbial communities
microbial community
decomposition
deamination
bacterium
degradation

Cite this

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title = "Effects of methyl bromide fumigation, chloropicrin fumigation and steam sterilization on soil nitrogen dynamics and microbial properties in a pot culture experiment",
abstract = "The effects of steam sterilization (SS), methyl bromide (MeBr) fumigation and chloropicrin (CP) fumigation on soil N dynamics and microbial properties were evaluated in a pot experiment. All disinfection treatments increased the -N level and inhibited nitrification. The additional -N in the CP treatment probably originated from the decomposition of microbial debris by surviving microbes, while that in the SS treatment was attributable to deamination processes of soil organic N occurring in a less labile fraction in addition to the decomposition of microbial debris. The MeBr fumigation increased the level of -N without changing the soil microbial biomass. Based on the determinations of soil microbial biomass, substrate utilization activity (Biolog method) and microbial community structure (phospholipid fatty acid method), the effects of the MeBr, CP and SS treatments on the microbial community were compared. The MeBr fumigation had relatively mild and short-term effects on microbial biomass and activity, but altered the community structure drastically by promoting the growth of gram-positive bacteria. The CP fumigation had large and long-term impacts on microbial biomass and activity; the community structure remained unaffected except for the gram-negative bacteria. Steam sterilization had severe and persistent effects on all parameters. The severity of the effects decreased in the order SS ≥ CP > MeBr. {\circledC} 2008 Japanese Society of Soil Science and Plant Nutrition.",
author = "T. Yamamoto and {Ultra Jr.}, V.U. and S. Tanaka and K. Sakurai and K. Iwasaki",
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Effects of methyl bromide fumigation, chloropicrin fumigation and steam sterilization on soil nitrogen dynamics and microbial properties in a pot culture experiment. / Yamamoto, T.; Ultra Jr., V.U.; Tanaka, S.; Sakurai, K.; Iwasaki, K.

In: Soil Science and Plant Nutrition, Vol. 54, No. 6, 2008, p. 886-894.

Research output: Contribution to journalArticle

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AU - Yamamoto, T.

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AU - Iwasaki, K.

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AB - The effects of steam sterilization (SS), methyl bromide (MeBr) fumigation and chloropicrin (CP) fumigation on soil N dynamics and microbial properties were evaluated in a pot experiment. All disinfection treatments increased the -N level and inhibited nitrification. The additional -N in the CP treatment probably originated from the decomposition of microbial debris by surviving microbes, while that in the SS treatment was attributable to deamination processes of soil organic N occurring in a less labile fraction in addition to the decomposition of microbial debris. The MeBr fumigation increased the level of -N without changing the soil microbial biomass. Based on the determinations of soil microbial biomass, substrate utilization activity (Biolog method) and microbial community structure (phospholipid fatty acid method), the effects of the MeBr, CP and SS treatments on the microbial community were compared. The MeBr fumigation had relatively mild and short-term effects on microbial biomass and activity, but altered the community structure drastically by promoting the growth of gram-positive bacteria. The CP fumigation had large and long-term impacts on microbial biomass and activity; the community structure remained unaffected except for the gram-negative bacteria. Steam sterilization had severe and persistent effects on all parameters. The severity of the effects decreased in the order SS ≥ CP > MeBr. © 2008 Japanese Society of Soil Science and Plant Nutrition.

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