Technology Transfer (T2) centres have emerged in many countries all over the world as effective agents and catalyst for improvements in disseminating experiences, technologies, best practices and innovations. There is a generally perceived link between the state of development of a country's transportation systems and the extent of technology usage and availability of technical information in that country. To keep the pace with the new technologies, the transportation sector needs information and knowledge that will enable it to advance their processes, incorporate new products into existing programmes, and increase technical know-how that produces positive change and economic development. This paper intended to discuss the innovative strategies adopted in operating transportation technology transfer centre in Botswana. Strategies adopted to achieve the objective were establishing and maintaining a transportation database and website accessible by transportation engineering professionals; publishing and disseminating quarterly T2 newsletter; hosting 6th Africa T2 conference; identifying training programs for the stakeholders. In conclusion, the paper highlighted the outcome of operating T2 centre to enhance transportation through effective technology transfer operations for economic development in Botswana.