Dynamics of pseudomonas aeruginosa azurin and its Cys3Ser mutant at single-crystal gold surfaces investigated by cyclic voltammetry and atomic force microscopy

Esben P. Friis, Jens E T Andersen, Lars L. Madsen, N. Bonander, Per Møller, Jens Ulstrup

    Research output: Contribution to journalArticle

    28 Citations (Scopus)

    Abstract

    Cyclic voltammetry of Pseudomonas aeruginosa azurin on polycrystalline gold is reversible (E0 = 360 mV vs she; 50 mM ammonium acetate) but the voltammetric signals decay with time constants of about 3 × 10-3 s-1. No signal is observed for monocrystalline Au(111). Cys3Ser azurin is electrochemically inactive on either type of gold electrode but shows a reversible although decaying peak (362 mV, 50 mM ammonium acetate; decay time constant ≈ 2 × 10-3 s-1) on edge-plane pyrolytic graphite. Ex situ and in situ atomic force microscopy (AFM) of the azurins on Au(111) show initially arrays of protein structures of lateral 100-200 Å and vertical ≈ 50 Å extension. These could be individual molecular images convoluted with the tip curvature. As scanning proceeds the structures in the ex situ mode collect into large two-dimensional assemblies while the adsorbed protein in the in situ mode is largely swept into the solution, recovering the free Au(111) surface. The cyclic voltammetry and AFM data are consistent with time dependent adsorption of the azurins on gold via the disulphide bridge (wild-type) or free thiol group (Cys3Ser mutant).

    Original languageEnglish
    Pages (from-to)2889-2897
    Number of pages9
    JournalElectrochimica Acta
    Volume42
    Issue number19
    DOIs
    Publication statusPublished - 1998

    Fingerprint

    Azurin
    Gold
    Cyclic voltammetry
    Atomic force microscopy
    Single crystals
    Proteins
    Graphite
    Sulfhydryl Compounds
    Disulfides
    Scanning
    Adsorption
    Electrodes

    All Science Journal Classification (ASJC) codes

    • Chemical Engineering(all)
    • Analytical Chemistry
    • Electrochemistry

    Cite this

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    title = "Dynamics of pseudomonas aeruginosa azurin and its Cys3Ser mutant at single-crystal gold surfaces investigated by cyclic voltammetry and atomic force microscopy",
    abstract = "Cyclic voltammetry of Pseudomonas aeruginosa azurin on polycrystalline gold is reversible (E0 = 360 mV vs she; 50 mM ammonium acetate) but the voltammetric signals decay with time constants of about 3 × 10-3 s-1. No signal is observed for monocrystalline Au(111). Cys3Ser azurin is electrochemically inactive on either type of gold electrode but shows a reversible although decaying peak (362 mV, 50 mM ammonium acetate; decay time constant ≈ 2 × 10-3 s-1) on edge-plane pyrolytic graphite. Ex situ and in situ atomic force microscopy (AFM) of the azurins on Au(111) show initially arrays of protein structures of lateral 100-200 {\AA} and vertical ≈ 50 {\AA} extension. These could be individual molecular images convoluted with the tip curvature. As scanning proceeds the structures in the ex situ mode collect into large two-dimensional assemblies while the adsorbed protein in the in situ mode is largely swept into the solution, recovering the free Au(111) surface. The cyclic voltammetry and AFM data are consistent with time dependent adsorption of the azurins on gold via the disulphide bridge (wild-type) or free thiol group (Cys3Ser mutant).",
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    Dynamics of pseudomonas aeruginosa azurin and its Cys3Ser mutant at single-crystal gold surfaces investigated by cyclic voltammetry and atomic force microscopy. / Friis, Esben P.; Andersen, Jens E T; Madsen, Lars L.; Bonander, N.; Møller, Per; Ulstrup, Jens.

    In: Electrochimica Acta, Vol. 42, No. 19, 1998, p. 2889-2897.

    Research output: Contribution to journalArticle

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    AU - Andersen, Jens E T

    AU - Madsen, Lars L.

    AU - Bonander, N.

    AU - Møller, Per

    AU - Ulstrup, Jens

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    AB - Cyclic voltammetry of Pseudomonas aeruginosa azurin on polycrystalline gold is reversible (E0 = 360 mV vs she; 50 mM ammonium acetate) but the voltammetric signals decay with time constants of about 3 × 10-3 s-1. No signal is observed for monocrystalline Au(111). Cys3Ser azurin is electrochemically inactive on either type of gold electrode but shows a reversible although decaying peak (362 mV, 50 mM ammonium acetate; decay time constant ≈ 2 × 10-3 s-1) on edge-plane pyrolytic graphite. Ex situ and in situ atomic force microscopy (AFM) of the azurins on Au(111) show initially arrays of protein structures of lateral 100-200 Å and vertical ≈ 50 Å extension. These could be individual molecular images convoluted with the tip curvature. As scanning proceeds the structures in the ex situ mode collect into large two-dimensional assemblies while the adsorbed protein in the in situ mode is largely swept into the solution, recovering the free Au(111) surface. The cyclic voltammetry and AFM data are consistent with time dependent adsorption of the azurins on gold via the disulphide bridge (wild-type) or free thiol group (Cys3Ser mutant).

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