Development of a real-time capacitive biosensor for cyclic cyanotoxic peptides based on Adda-specific antibodies

Lesedi Lebogang, Martin Hedström, Bo Mattiasson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The harmful effects of cyanotoxins in surface waters have led to increasing demands for accurate early warning methods. This study proposes a capacitive immunosensor for broad-spectrum detection of the group of toxic cyclic peptides called microcystins (~80 congeners) at very low concentration levels. The novel analytical platform offers significant advances compared to the existing methods. Monoclonal antibodies (mAbs, clone AD4G2) that recognize a common element of microcystins were used to construct the biosensing layer. Initially, a stable insulating anchor layer for the mAbs was made by electropolymerization of tyramine onto a gold electrode surface, with subsequent incorporation of gold nanoparticles (AuNPs) on the glutaraldehyde (5%) activated polytyramine surface. The biosensor responded linearly to microcystin concentrations from 1×10-13M to 1×10-10M MC-LR standard with a limit of detection of 2.1×10-14M. The stability of the biosensor was evaluated by repeated measurements of the antigen and by determining the capacitance change relative to the original response, which decreased below 90% after the 30th cycle.

Original languageEnglish
Pages (from-to)69-76
Number of pages8
JournalAnalytica Chimica Acta
Volume826
Issue number1
DOIs
Publication statusPublished - Jan 1 2014

Fingerprint

Microcystins
Cyclic Peptides
Biosensing Techniques
Biosensors
Gold
peptide
antibody
gold
Immunosensors
Tyramine
Electropolymerization
Antibodies
Poisons
Glutaral
antigen
Anchors
anchor
Surface waters
Nanoparticles
Limit of Detection

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

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abstract = "The harmful effects of cyanotoxins in surface waters have led to increasing demands for accurate early warning methods. This study proposes a capacitive immunosensor for broad-spectrum detection of the group of toxic cyclic peptides called microcystins (~80 congeners) at very low concentration levels. The novel analytical platform offers significant advances compared to the existing methods. Monoclonal antibodies (mAbs, clone AD4G2) that recognize a common element of microcystins were used to construct the biosensing layer. Initially, a stable insulating anchor layer for the mAbs was made by electropolymerization of tyramine onto a gold electrode surface, with subsequent incorporation of gold nanoparticles (AuNPs) on the glutaraldehyde (5{\%}) activated polytyramine surface. The biosensor responded linearly to microcystin concentrations from 1×10-13M to 1×10-10M MC-LR standard with a limit of detection of 2.1×10-14M. The stability of the biosensor was evaluated by repeated measurements of the antigen and by determining the capacitance change relative to the original response, which decreased below 90{\%} after the 30th cycle.",
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Development of a real-time capacitive biosensor for cyclic cyanotoxic peptides based on Adda-specific antibodies. / Lebogang, Lesedi; Hedström, Martin; Mattiasson, Bo.

In: Analytica Chimica Acta, Vol. 826, No. 1, 01.01.2014, p. 69-76.

Research output: Contribution to journalArticle

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