Development of a real-time capacitive biosensor for cyclic cyanotoxic peptides based on Adda-specific antibodies

Lesedi Lebogang, Martin Hedström, Bo Mattiasson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The harmful effects of cyanotoxins in surface waters have led to increasing demands for accurate early warning methods. This study proposes a capacitive immunosensor for broad-spectrum detection of the group of toxic cyclic peptides called microcystins (~80 congeners) at very low concentration levels. The novel analytical platform offers significant advances compared to the existing methods. Monoclonal antibodies (mAbs, clone AD4G2) that recognize a common element of microcystins were used to construct the biosensing layer. Initially, a stable insulating anchor layer for the mAbs was made by electropolymerization of tyramine onto a gold electrode surface, with subsequent incorporation of gold nanoparticles (AuNPs) on the glutaraldehyde (5%) activated polytyramine surface. The biosensor responded linearly to microcystin concentrations from 1×10-13M to 1×10-10M MC-LR standard with a limit of detection of 2.1×10-14M. The stability of the biosensor was evaluated by repeated measurements of the antigen and by determining the capacitance change relative to the original response, which decreased below 90% after the 30th cycle.

Original languageEnglish
Pages (from-to)69-76
Number of pages8
JournalAnalytica Chimica Acta
Volume826
Issue number1
DOIs
Publication statusPublished - Jan 1 2014

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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