Cytochrome c dynamics at gold and glassy carbon surfaces monitored by in situ scanning tunnel microscopy

Jens E.T. Andersen, Per Møller, Marianne V. Pedersen, Jens Ulstrup

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Abstract

We have investigated the absorption of cytochrome c on gold and glassy carbon substrates by in situ scanning tunnel microscopy under potentiostatic control of both substrate and tip. Low ionic strength and potential ranges where no Faradaic current flows were used. Cyt c aggregates into flat composite structures of about 50 nm lateral extension at gold surfaces. The aggregates evolve in time, and structures resembling individual cyt c molecules can be distinguished in the space between the 50 nm structures. Cyt c aggregates also form at glassy carbon but have a different, unbroken character where cyt c both sticks well to the surface and exhibits notable mobility. The observations suggest that characteristic surface specific, internally mobile protein aggregates are formed at both surfaces and that in situ molecular resolution of the STM pictures may have been achieved.

Original languageEnglish
Pages (from-to)193-205
Number of pages13
JournalSurface Science
Volume325
Issue number1-2
DOIs
Publication statusPublished - Feb 20 1995

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All Science Journal Classification (ASJC) codes

  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Materials Chemistry

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