Comparison of vitronectin and fibronectin as substrates during the culture of human embryonic stem cells

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Abstract

The feeder free culturing of pluripotent stem cells aims to eliminate
the use of mouse embryonic fibroblasts (MEFs) as substrate during
in vitro culturing. Here, the potential of fibronectin and vitronectin to
keep stem cells undifferentiated was compared using two different
stem cell lines (HUES1 and HUES7). Cells were cultured on
vitronectin and fibronectin for 3 weeks. Results showed that cells
cultured in fibronectin appear to adhere slightly well initially while
those in vitronectin detached and bulked up in culture. However,
cells cultured on either substrate were well attached and expressed
Oct4 and Nanog at the end of the 3-week protocols. This signals
the capacity of both vitronectin and fibronectin to keep stem cells
undifferentiated after a few passages. Such comparisons of stem
cell culture substrates allow the maximization of stem cell culture
conditions that are specific to the cell line in use.
Original languageEnglish
Number of pages4
JournalJournal of Regenerative Medicine
Volume9
Issue number3
DOIs
Publication statusPublished - 2020

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