Characterization and comparative analysis of antibiotic resistance plasmids isolated from a wastewater treatment plant

Teddie O. Rahube, Laia Santiña Viana, Günther Koraimann, Christopher K. Yost

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    A wastewater treatment plant (WWTP) is an environment high in nutrient concentration with diverse bacterial populations and can provide an ideal environment for the proliferation of mobile elements such as plasmids. WWTPs have also been identified as reservoirs for antibiotic resistance genes that are associated with human pathogens. The objectives of this study were to isolate and characterize self-transmissible or mobilizable resistance plasmids associated with effluent from wastewater treatment plant. An enrichment culture approach designed to capture plasmids conferring resistance to high concentrations of erythromycin was used to capture plasmids from an urban wastewater treatment plant servicing a population of ca. 210,000. DNA sequencing of the plasmids revealed diversity of plasmids represented by incompatibility groups IncU, col-E, IncFII and IncP-1β. Genes coding resistance to clinically relevant antibiotics (macrolide, tetracycline, beta-lactam, trimethoprim, chloramphenicol, sulphonamide), quaternary ammonium compounds and heavy metals were co-located on these plasmids, often within transposable and integrative mobile elements. Several of the plasmids were self-transmissible or mobilizable and could be maintained in the absence of antibiotic selection. The IncFII plasmid pEFC36a showed the highest degree of sequence identity to plasmid R1 which has been isolated in England more than fifty years ago from a patient suffering from a Salmonella infection. Functional conservation of key regulatory features of this F-like conjugation module were demonstrated by the finding that the conjugation frequency of pEFC36a could be stimulated by the positive regulator of plasmid R1 DNA transfer genes, TraJ.

    Original languageEnglish
    Article number558
    JournalFrontiers in Microbiology
    Volume5
    Issue numberOCT
    DOIs
    Publication statusPublished - 2014

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    Microbial Drug Resistance
    Waste Water
    Plasmids
    Therapeutics
    Quaternary Ammonium Compounds
    Genes
    Anti-Bacterial Agents
    Trimethoprim
    Salmonella Infections
    Sulfonamides
    Macrolides
    beta-Lactams
    Chloramphenicol
    Erythromycin
    Heavy Metals
    Tetracycline
    DNA Sequence Analysis
    England
    Population
    Food

    All Science Journal Classification (ASJC) codes

    • Microbiology
    • Microbiology (medical)

    Cite this

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    Characterization and comparative analysis of antibiotic resistance plasmids isolated from a wastewater treatment plant. / Rahube, Teddie O.; Viana, Laia Santiña; Koraimann, Günther; Yost, Christopher K.

    In: Frontiers in Microbiology, Vol. 5, No. OCT, 558, 2014.

    Research output: Contribution to journalArticle

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