CaCO3 precipitation in selected bacteria mixture by activated microbial induction

Emmanuel Emem Obong Agbenyeku, Edison Muzenda, Innocent Mandla Msibi

    Research output: Contribution to conferencePaper

    Abstract

    Activated microbial induction of CaCO3precipitation (AMICP) is a niche with innovative approaches that involves the exploration of bacterial attacks towards the alteration/transformation of the physical properties of soils. A handful have been documented on this technology in developed countries however, much is yet to be understood in developing African countries like South Africa, just as vermicomposting and waste water treatment technologies are gaining grounds. It is widely known that AMICP by urea hydrolysis in natural soils is possibly affected by contacts between ureolytic and non-ureolytic bacteria, the study explored a designed and experimental assessment of the relations between ureolytic and nonureolytic bacteria and their interactive effects on AMICP. Through existing studies an artificial leveled groundwater medium was injected with model species of bacteria i.e., ureolytic species Sporosarcina pasteurii and the non-ureolytic species Bacillus subtilis. The control treatment was inoculated with a pure culture of S. pasteurii under measurements of pH, optical density (OD), development of NH4-N, dissolved calcium (DC) and dissolved inorganic carbon (DIC). Outcomes revealed DC precipitated as CaCO3slower in the control mixture than in the mixed culture irrespective of unfavorable conditions in the mixed culture, i.e., lower concentrations of pH and CO3 2-. Higher density of bacterial cells in the mixed culture resulted from B. subtilis showing significant higher growth rate than S. pasteurii. Previous authors indicate that the presence of the non-ureolytic bacterial species, B. subtilis, stimulate AMICP process through supply of nucleation sites in the form of non-ureolytic bacterial cells.

    Original languageEnglish
    Pages543-547
    Number of pages5
    Publication statusPublished - 2016
    EventWorld Congress on Engineering 2016, WCE 2016 - London, United Kingdom
    Duration: Jun 29 2016Jul 1 2016

    Other

    OtherWorld Congress on Engineering 2016, WCE 2016
    CountryUnited Kingdom
    CityLondon
    Period6/29/167/1/16

    Fingerprint

    Bacteria
    Calcium
    Soils
    Density (optical)
    Bacilli
    Water treatment
    Developing countries
    Urea
    Groundwater
    Hydrolysis
    Wastewater
    Nucleation
    Physical properties
    Carbon

    All Science Journal Classification (ASJC) codes

    • Computer Science (miscellaneous)

    Cite this

    Agbenyeku, E. E. O., Muzenda, E., & Msibi, I. M. (2016). CaCO3 precipitation in selected bacteria mixture by activated microbial induction. 543-547. Paper presented at World Congress on Engineering 2016, WCE 2016, London, United Kingdom.
    Agbenyeku, Emmanuel Emem Obong ; Muzenda, Edison ; Msibi, Innocent Mandla. / CaCO3 precipitation in selected bacteria mixture by activated microbial induction. Paper presented at World Congress on Engineering 2016, WCE 2016, London, United Kingdom.5 p.
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    abstract = "Activated microbial induction of CaCO3precipitation (AMICP) is a niche with innovative approaches that involves the exploration of bacterial attacks towards the alteration/transformation of the physical properties of soils. A handful have been documented on this technology in developed countries however, much is yet to be understood in developing African countries like South Africa, just as vermicomposting and waste water treatment technologies are gaining grounds. It is widely known that AMICP by urea hydrolysis in natural soils is possibly affected by contacts between ureolytic and non-ureolytic bacteria, the study explored a designed and experimental assessment of the relations between ureolytic and nonureolytic bacteria and their interactive effects on AMICP. Through existing studies an artificial leveled groundwater medium was injected with model species of bacteria i.e., ureolytic species Sporosarcina pasteurii and the non-ureolytic species Bacillus subtilis. The control treatment was inoculated with a pure culture of S. pasteurii under measurements of pH, optical density (OD), development of NH4-N, dissolved calcium (DC) and dissolved inorganic carbon (DIC). Outcomes revealed DC precipitated as CaCO3slower in the control mixture than in the mixed culture irrespective of unfavorable conditions in the mixed culture, i.e., lower concentrations of pH and CO3 2-. Higher density of bacterial cells in the mixed culture resulted from B. subtilis showing significant higher growth rate than S. pasteurii. Previous authors indicate that the presence of the non-ureolytic bacterial species, B. subtilis, stimulate AMICP process through supply of nucleation sites in the form of non-ureolytic bacterial cells.",
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    Agbenyeku, EEO, Muzenda, E & Msibi, IM 2016, 'CaCO3 precipitation in selected bacteria mixture by activated microbial induction' Paper presented at World Congress on Engineering 2016, WCE 2016, London, United Kingdom, 6/29/16 - 7/1/16, pp. 543-547.

    CaCO3 precipitation in selected bacteria mixture by activated microbial induction. / Agbenyeku, Emmanuel Emem Obong; Muzenda, Edison; Msibi, Innocent Mandla.

    2016. 543-547 Paper presented at World Congress on Engineering 2016, WCE 2016, London, United Kingdom.

    Research output: Contribution to conferencePaper

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    AU - Msibi, Innocent Mandla

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    Agbenyeku EEO, Muzenda E, Msibi IM. CaCO3 precipitation in selected bacteria mixture by activated microbial induction. 2016. Paper presented at World Congress on Engineering 2016, WCE 2016, London, United Kingdom.