Antioxidant properties and anti-atherogenic effects of corn fractions in LDL receptor knockout mice.

Kabo Masisi, Khuong Le, William Diehl-Jones, Mohammed H Moghadasian, Trust Beta

Research output: Contribution to journalArticlepeer-review

Abstract

Excess reactive oxygen species (ROS) produced in vivo may not be
adequately counteracted by endogenous antioxidant systems leading
to harmful modifications of lipids, proteins, carbohydrates and nucleic
acids. Such alterations may result in abnormal cell function; this
occurrence is known as oxidative stress. Several studies have suggested
that oxidative stress is one of the causative factors in pathogenesis
of chronic diseases. It has been reported that dietary antioxidant
are associated with reduced chronic diseases. Our previous study investigated
the antioxidant potential of carotenoid extracts of handseparated
aleurone, germ and endosperm fractions of barley, corn and
wheat. The data showed significantly higher (P<0.001) antioxidant
activity in the germ than in the aleurone and endosperm fractions.
Using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide
(MTT) assay, 2,2=azobis (2-amidinopropane) dihydrochloride (AAPH)-
induced cell loss was effectively reduced by pre-incubating Caco-2,
HT-29 and FHs 74 Int cells with carotenoid extracts. Moreover, carotenoid
extracts reduced (P<0.001) AAPH-induced intracellular oxidation
in the cell lines, suggesting significant antioxidant activities.
Based on previous results, it is hypothesized that corn botanical fractions
will prevent LDL oxidation hence mitigating atherosclerosis. To
test this hypothesis, the current study is being carried out to investigate
anti-atherogenic effects of hand-separated corn fractions (aleurone,
endosperm and germ) in LDL receptor knockout (LDL-r-KO)
mice. Four groups of male LDL-r-KO mice are fed with the experimental
diets supplemented with (3 treated groups) or without (1 control
group) 5% (w/w) of each of corn fraction for 10 weeks. All diets were
supplemented with 0.06% (w/w) dietary cholesterol. LDL-r-KO mice are
well established mice models for studying LDL oxidation and atherosclerosis.
This study, approved by the Animal Care Committee at the
University of Manitoba, is currently in progress. Blood lipid modifications
levels, body weight and food intake will be measured every four
weeks. Susceptibility of LDL particles to Cu-induced oxidation as well
as the extent and degree of atherogenesis will be examined at the end
of the study. This study will have significant contribution to LDL oxidation
and will encourage further investigations of potential cardiovascular
benefits of cereal grain fractions. (Supported by a Canada
Research Chair grant.)
Original languageEnglish
Article number40
Pages (from-to)518
Number of pages1
JournalApplied Physiology, Nutrition and Metabolism
Volume40
Issue number5
Publication statusPublished - May 30 2015

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