Analysis of crystal structures of LXR beta in relation to plasticity of the ligand-binding domain upon ligand binding

Nagullapailli Mallini, Hariharan Rajesh, Pooja Berwal, Samiron Phukan, Vittukudi Narayanaiyengar Balaji

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Liver X receptors (LXRs) are a member of the nuclear hormone receptor superfamilly of ligand activated transcription factors. LXRs have gained importance as therapeutic targets because of their involvement in many diseases. Analysis of the protein-ligand complexes of X-ray crystallography-derived structures revealed that residues His435 and Trp457 act as a switch that mediates ligand activation. These residues show conservation for main chain (phi, psi) in His435 and moderate movement for Trp457. Hiis435 in Hellix11 (H11) is conserved in relation to Trp457 in Helix12 (H12). This shows that some induced fit effect can be incorporated while designing ligands for activation of LXR with relation to Trp457 rather than that of His435. Similarly, main chain movement in Phe329 and Leu330 showed significant conformational mobility leading to flexibility in the ligand-binding domain (LBD) along with Arg319 which has a moderate movement in (phi and psi) angles. It is interesting to know that for some sequence-ligand complex crystallizations using different conditions show considerable main chain and side chain mobility indicating plasticity in LBD of LXR beta. This study supports our understanding the relative movement of residues in the Ill of LXRs upon ligand binding which can provide insight for designing of LXRs modulators.
Original languageEnglish
Pages (from-to)140-154
Number of pages15
JournalChemical Biology and Drug Design
Volume71
Issue number2
Publication statusPublished - 2008

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Liver
Plasticity
Crystal structure
Ligands
Chemical activation
Liver X Receptors
X ray crystallography
X Ray Crystallography
Cytoplasmic and Nuclear Receptors
Crystallization
Modulators
Conservation
Transcription Factors
Switches
Proteins

Cite this

Mallini, Nagullapailli ; Rajesh, Hariharan ; Berwal, Pooja ; Phukan, Samiron ; Balaji, Vittukudi Narayanaiyengar. / Analysis of crystal structures of LXR beta in relation to plasticity of the ligand-binding domain upon ligand binding. In: Chemical Biology and Drug Design. 2008 ; Vol. 71, No. 2. pp. 140-154.
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abstract = "Liver X receptors (LXRs) are a member of the nuclear hormone receptor superfamilly of ligand activated transcription factors. LXRs have gained importance as therapeutic targets because of their involvement in many diseases. Analysis of the protein-ligand complexes of X-ray crystallography-derived structures revealed that residues His435 and Trp457 act as a switch that mediates ligand activation. These residues show conservation for main chain (phi, psi) in His435 and moderate movement for Trp457. Hiis435 in Hellix11 (H11) is conserved in relation to Trp457 in Helix12 (H12). This shows that some induced fit effect can be incorporated while designing ligands for activation of LXR with relation to Trp457 rather than that of His435. Similarly, main chain movement in Phe329 and Leu330 showed significant conformational mobility leading to flexibility in the ligand-binding domain (LBD) along with Arg319 which has a moderate movement in (phi and psi) angles. It is interesting to know that for some sequence-ligand complex crystallizations using different conditions show considerable main chain and side chain mobility indicating plasticity in LBD of LXR beta. This study supports our understanding the relative movement of residues in the Ill of LXRs upon ligand binding which can provide insight for designing of LXRs modulators.",
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Analysis of crystal structures of LXR beta in relation to plasticity of the ligand-binding domain upon ligand binding. / Mallini, Nagullapailli; Rajesh, Hariharan; Berwal, Pooja; Phukan, Samiron; Balaji, Vittukudi Narayanaiyengar.

In: Chemical Biology and Drug Design, Vol. 71, No. 2, 2008, p. 140-154.

Research output: Contribution to journalArticle

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T1 - Analysis of crystal structures of LXR beta in relation to plasticity of the ligand-binding domain upon ligand binding

AU - Mallini, Nagullapailli

AU - Rajesh, Hariharan

AU - Berwal, Pooja

AU - Phukan, Samiron

AU - Balaji, Vittukudi Narayanaiyengar

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AB - Liver X receptors (LXRs) are a member of the nuclear hormone receptor superfamilly of ligand activated transcription factors. LXRs have gained importance as therapeutic targets because of their involvement in many diseases. Analysis of the protein-ligand complexes of X-ray crystallography-derived structures revealed that residues His435 and Trp457 act as a switch that mediates ligand activation. These residues show conservation for main chain (phi, psi) in His435 and moderate movement for Trp457. Hiis435 in Hellix11 (H11) is conserved in relation to Trp457 in Helix12 (H12). This shows that some induced fit effect can be incorporated while designing ligands for activation of LXR with relation to Trp457 rather than that of His435. Similarly, main chain movement in Phe329 and Leu330 showed significant conformational mobility leading to flexibility in the ligand-binding domain (LBD) along with Arg319 which has a moderate movement in (phi and psi) angles. It is interesting to know that for some sequence-ligand complex crystallizations using different conditions show considerable main chain and side chain mobility indicating plasticity in LBD of LXR beta. This study supports our understanding the relative movement of residues in the Ill of LXRs upon ligand binding which can provide insight for designing of LXRs modulators.

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